DNA

Part:BBa_K1971003:Design

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-20)


Mal85 gene with BsaI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI.rc site found at 1382


Design Notes

be sure to optimized in order to eliminate the EcorI site


Source

artificial sequence

References